The use of enzyme-linked immunosorbent assay systems for serology and antigen detection in parvovirus, coronavirus and rotavirus infections in dogs in The Netherlands
Identifieur interne : 001B71 ( Main/Exploration ); précédent : 001B70; suivant : 001B72The use of enzyme-linked immunosorbent assay systems for serology and antigen detection in parvovirus, coronavirus and rotavirus infections in dogs in The Netherlands
Auteurs : G. F. Rimmelzwaan [Pays-Bas] ; J. Groen [Pays-Bas] ; H. Egberink [Pays-Bas] ; G. H. A. Borst [Pays-Bas] ; F. G. C. M. Uytdehaag [Pays-Bas] ; A. D. M. E. Osterhaus [Pays-Bas]Source :
- Veterinary Microbiology [ 0378-1135 ] ; 1991.
English descriptors
- Teeft :
- Acute diarrhea, Antibody, Antibody titers, Antigen detection, Antigen elisa, Antigen elisas, Antigenic relationship, Antiviral antibodies, Assay, Biotin, Canine, Canine parvovirus, Carbonate buffer, Coronavirus, Diarrhea, Elisa, Elisa systems, Fecal, Fecal samples, Feces, Gastroenteritis, Gastrointestinal disease, Groen, Immunosorbent, Immunosorbent assay, Immunosorbent assays, Indirect elisa, Indirect elisa system, Kennel, Major cause, Microtiter plates, Monoclonal, Monoclonal antibodies, Negative results, Netherlands, Open population, Osterhaus, Overall correlation, Parvovirus, Peritonitis virus, Problem kennels, Recurrent problems, Respective viruses, Rimmelzwaan, Rotavirus, Rotavirus antigen, Rotavirus antigens, Rotavirus infections, Serological elisa systems, Serum antibodies, Serum samples, Titer, Vaccination, Viral, Viral antigens, Viral gastroenteritis.
Abstract
Abstract: Complex trapping blocking (CTB) enzyme-linked immunosorbent assays (ELISAs) and indirect ELISAs for the detection of antibodies to canine parvovirus (CPV), canine coronavirus (CCV) and rotavirus in sera of dogs were established. Double antibody sandwich ELISAs for the detection of CPV-, CCV- and rotavirus antigens in fecal samples were also developed. Both the serological and antigen-detection ELISAs were used to screen samples from dogs in The Netherlands, with or without a history of acute diarrhea. It was shown that the results of the respective serological ELISAs correlated well and that CPV was the major cause of virus-induced acute diarrhea in dogs in The Netherlands.
Url:
DOI: 10.1016/0378-1135(91)90039-I
Affiliations:
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Egberink</name><affiliation wicri:level="1"><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Department of Virology, Veterinary Faculty, State University of Utrecht, Yalelaan 1, 3508 TD Utrecht</wicri:regionArea><wicri:noRegion>3508 TD Utrecht</wicri:noRegion></affiliation></author><author><name sortKey="Borst, G H A" sort="Borst, G H A" uniqKey="Borst G" first="G. H. A." last="Borst">G. H. A. Borst</name><affiliation wicri:level="1"><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Regional animal Health Service Centre, Zwartewaterallee 8, 8031 DX Zwolle</wicri:regionArea><wicri:noRegion>8031 DX Zwolle</wicri:noRegion></affiliation></author><author><name sortKey="Uytdehaag, F G C M" sort="Uytdehaag, F G C M" uniqKey="Uytdehaag F" first="F. G. C. M." last="Uytdehaag">F. G. C. M. Uytdehaag</name><affiliation wicri:level="1"><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Laboratory of Immunobiology, National Institute of Public Health and Environmental Protection, P.O. Box 1, 3720 BA Bilthoven</wicri:regionArea><wicri:noRegion>3720 BA Bilthoven</wicri:noRegion></affiliation></author><author><name sortKey="Osterhaus, A D M E" sort="Osterhaus, A D M E" uniqKey="Osterhaus A" first="A. D. M. E." last="Osterhaus">A. D. M. E. Osterhaus</name><affiliation wicri:level="1"><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Laboratory of Immunobiology, National Institute of Public Health and Environmental Protection, P.O. Box 1, 3720 BA Bilthoven</wicri:regionArea><wicri:noRegion>3720 BA Bilthoven</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j">Veterinary Microbiology</title><title level="j" type="abbrev">VETMIC</title><idno type="ISSN">0378-1135</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1991">1991</date><biblScope unit="volume">26</biblScope><biblScope unit="issue">1–2</biblScope><biblScope unit="page" from="25">25</biblScope><biblScope unit="page" to="40">40</biblScope></imprint><idno type="ISSN">0378-1135</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0378-1135</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Acute diarrhea</term><term>Antibody</term><term>Antibody titers</term><term>Antigen detection</term><term>Antigen elisa</term><term>Antigen elisas</term><term>Antigenic relationship</term><term>Antiviral antibodies</term><term>Assay</term><term>Biotin</term><term>Canine</term><term>Canine parvovirus</term><term>Carbonate buffer</term><term>Coronavirus</term><term>Diarrhea</term><term>Elisa</term><term>Elisa systems</term><term>Fecal</term><term>Fecal samples</term><term>Feces</term><term>Gastroenteritis</term><term>Gastrointestinal disease</term><term>Groen</term><term>Immunosorbent</term><term>Immunosorbent assay</term><term>Immunosorbent assays</term><term>Indirect elisa</term><term>Indirect elisa system</term><term>Kennel</term><term>Major cause</term><term>Microtiter plates</term><term>Monoclonal</term><term>Monoclonal antibodies</term><term>Negative results</term><term>Netherlands</term><term>Open population</term><term>Osterhaus</term><term>Overall correlation</term><term>Parvovirus</term><term>Peritonitis virus</term><term>Problem kennels</term><term>Recurrent problems</term><term>Respective viruses</term><term>Rimmelzwaan</term><term>Rotavirus</term><term>Rotavirus antigen</term><term>Rotavirus antigens</term><term>Rotavirus infections</term><term>Serological elisa systems</term><term>Serum antibodies</term><term>Serum samples</term><term>Titer</term><term>Vaccination</term><term>Viral</term><term>Viral antigens</term><term>Viral gastroenteritis</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Complex trapping blocking (CTB) enzyme-linked immunosorbent assays (ELISAs) and indirect ELISAs for the detection of antibodies to canine parvovirus (CPV), canine coronavirus (CCV) and rotavirus in sera of dogs were established. Double antibody sandwich ELISAs for the detection of CPV-, CCV- and rotavirus antigens in fecal samples were also developed. Both the serological and antigen-detection ELISAs were used to screen samples from dogs in The Netherlands, with or without a history of acute diarrhea. It was shown that the results of the respective serological ELISAs correlated well and that CPV was the major cause of virus-induced acute diarrhea in dogs in The Netherlands.</div></front></TEI><affiliations><list><country><li>Pays-Bas</li></country></list><tree><country name="Pays-Bas"><noRegion><name sortKey="Rimmelzwaan, G F" sort="Rimmelzwaan, G F" uniqKey="Rimmelzwaan G" first="G. F." last="Rimmelzwaan">G. F. Rimmelzwaan</name></noRegion><name sortKey="Borst, G H A" sort="Borst, G H A" uniqKey="Borst G" first="G. H. A." last="Borst">G. H. A. Borst</name><name sortKey="Egberink, H" sort="Egberink, H" uniqKey="Egberink H" first="H." last="Egberink">H. Egberink</name><name sortKey="Groen, J" sort="Groen, J" uniqKey="Groen J" first="J." last="Groen">J. Groen</name><name sortKey="Osterhaus, A D M E" sort="Osterhaus, A D M E" uniqKey="Osterhaus A" first="A. D. M. E." last="Osterhaus">A. D. M. E. Osterhaus</name><name sortKey="Uytdehaag, F G C M" sort="Uytdehaag, F G C M" uniqKey="Uytdehaag F" first="F. G. C. M." last="Uytdehaag">F. G. C. M. Uytdehaag</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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